Period
2019 - 2022
Colaborators
Responsible: Dr. Insung Park and Dr. Sergio Navarro-Serna
PI: Dr. Pablo Ross, University of Californa, Davis, CA - United States
PI: Dr. Joaquin Gadea, University of Murcia, Murcia , Spain.
Colaborator: Dr.Elizabeth Maga, University of Californa, Davis, CA - United States
Colaborator: Dr. Raquel Pinho, University of Californa, Davis, CA - United States
Colaborator: Sergio Navarro-Serna, University of Murcia, Murcia , Spain.
Colaborator: Dr. Seung Kim, Stanford University School of Medicine, Stanford, CA - United States .
Location
- University of California, Davis, CA - United States
Raquel Pinho’s main responsabilities
- Genotyping of pig embryos and fetuses at on-target and off-target sites by PacBio SMRT Sequencing.
- PacBio data processing in linux
- PacBio data analysis in R
- PacBio data visualization in R
Description
In this project, we used the CRISPR/Cas9 system targeting a porcine gene in zygotes using different delivery conditions. Cleavage efficiency, mutation frequency and off-target effects were analyzed, first with in vivo using blastocysts and parthenotes. We used PacBio sequencing to analyze, also mutation and mosaicism rate with amplicon deep sequencing.
Data
Data not published yet
Publications
Data not published yet