Profile
Molecular biologist with more than 10 years of laboratory experience , working in a range of projects from target genetic modification to integration of multiple omics datasets. Thriving in multicultural and multidisciplinary environments, I have worked in different teams, on supervision, management and training. I enjoy learning new skills and technologies, having learned programming, including R package development and dashboard design. I am motivated by challenges and willing to learn and share knowledge.
Experience
| Molecular Biology | Programming | Data analysis |
|---|---|---|
| PCR | R | RNAseq data processing |
| qPCR | Rmarkdown | RNAseq data analysis |
| RTPCR | Shiny | RNAseq data visualization |
| ddPCR (QX200) | Dashboards(Shiny) | Amplicon sequencig analysis |
| rhAmpSeq | Linux shell | Amplicon deep sequencig analysis (Illumina/PacBio) |
| CHANGE-Seq | Slurm | Statistical analysis |
| DNA extraction | SQL | Data visualization |
| RNA extraction | Python | DNA methylation analysis |
| Cloning | Jupyter notebook | |
| Bacterial transformation | Web scraping | |
| Cellular transfection | R packages | |
| Sanger sequencing | ||
| RFLP | ||
| CRISPR/Cas9 system | ||
| Genotyping | ||
| Library construction (NGS) | ||
| Western blot | ||
| Southern blot | ||
| Mammalian cell culture | ||
| Oocyte collection | ||
| Protein extraction |
Education
PhD in Animal Science at University of California, Davis, CA. December 2019.
Master of Science at School of Veterinary Medicine and Animal Science of the University of São Paulo, SP - Brazil. September 2014.
Bachelor’s degree in physicaltherapy at University of Fortaleza, CE - Brazil. December 2011.
Research
Post-Doctoral Researcher, University of California, Davis January 2020 – present. Main responsabilities and projects: • Developing methodology for detection of off-target events of CRISPR/Cas9 system in porcine model. • Pig oocyte collection, maturation and activation for development into parthenotes and posterior electroporation of CRISPR/Cas9 components. • PCR of possible off-target sites for Cas9 in vitro assay experiments • Genotyping of pig embryos and fetuses at on-target and off-target sites by sanger sequencing and PacBio SMRT Sequencing. • Assay development for guideRNA/Cas9 efficiency • Development of multiplex rhAmp PCR panel for analysis off-target sites. • Management of molecular biology laboratory.
Post-Doctoral Researcher, GeneTether (Sector: Biotechnology) July 2021 – present. Main responsabilities and projects: • Development of ddPCR and qPCR assay for testing efficiency of CRISPR/Cas9 system in bESC and porcine fibroblasts. • Development of template to be used in HDR driven by modified CRISPR/tCas9 in bESC and porcine embryos. • Cloning and stock plasmids and genetic templates
Doctoral Researcher, University of California, Davis Department of Animal Science. September 2014 – December 2019 Main responsabilities and projects: • Experiment design and project writing. • Library construction for RNA-sequencing and DNA methylation experiments. • RNA-seq and DNA methylation data processing in Linux. • RNA-seq and DNA methylation data analysis using R. • Weighted co-expression network analysis using WGCNA. • Integration of intestinal transcriptome, hepatic metabolome and intestinal microbiota 16S data using MixOmics. • Validation of NGS results using qPCR for relative gene expression analysis.
Master Researcher, University of São Paulo, São Paulo January 2012 – August 2014 Main responsabilities and projects: • Design and construction of DNA template for homologous direct repair of dystrophin gene in dogs using PCR for homology arms amplification. • Design and assembly of pairs of Transcription Activator-Like Effector Nucleases (TALENS) targeting canine dystrophin gene by Golden Gate platform. • Design and construction of siRNA targeting the Ku70 transcript. • Silencing of the genes Ku70, ATP7IP and EP300 using siRNA in MDCK cells for enhanced gene editing of dystrophin gene by TALENs.
Undergrad Researcher | University of Fortaleza, Fortaleza. March 2011 – February 2012 | Main responsabilities and projects: ·Learning and using techniques such as PCR, cloning, southern blot, western blot, qPCR , cell culture for different projects. ·Use of qPCR for gene copy number analysis in goat transgenic cell lines. ·Isolation of monocytes from goat blood samples for cell culture using Ficoll- Hypaque method for posterior study of interaction of those cells with Mycobacterium Avium ssp paratuberculosis.
Courses
·Data Intensive Biology Summer Institute (DIBSI) ANGUS workshop. University of California, June 26th to July 8th 2017. ·Introuduction to SQL Server/ SQL for joining data/ Data manipulation in SQL/ Intermediate SQL queries, DataCamp 2021/2022 ·Exploratory Data Analysis in Python/ Intermediate Python/ Python Data Science Toolbox, DataCamp 2021 ·Understanding Data visualization/ Introduction to tidyverse. DataCamp 2021